Glycogen accumulation of wild-type and mutant yeast cells with a disrupted GRAP homologue.

Five tetrads dissected from heterozygous diploids for thegrhδ1::URA3 allele were patched on the YED-2% glucose plate, incubated at 30°C overnight, and stained with iodine vapor. Dark-stained patches contain cells positive for glycogen accumulation as well as the URA3 marker (data not shown).