Pulse-chase analysis of dimerization of rVWFR273W in COS-7 cells.

COS-7 cells were transfected with the appropriate plasmid DNA and pulse-chased as in Figure 3. Radiolabeled cell lysates were immunoprecipitated with a rabbit polyclonal antiserum to VWF. Immunoprecipitates were divided into 2 aliquots, then separated by electrophoresis on 6% reducing and nonreducing SDS-polyacrylamide gels. Upper bar denotes the position of pro-VWF dimers, lower bar denotes the position of pro-VWF monomers.