Fig. 3.
![Fig. 3. Pulse-chase analysis of rVWFR273W secretion from COS-7 cells. / COS-7 cells were transfected with the appropriate plasmid DNA. Forty-eight hours after transfection, the cells were radiolabeled for 20 minutes with [35S]-methionine and [35S]-cysteine, then chased for various times in the presence of nonradioactive methionine and cysteine. Radiolabeled cell lysates and medium samples were immunoprecipitated with a rabbit polyclonal antiserum to VWF. Immunoprecipitates were separated by electrophoresis on 6% reducing SDS-polyacrylamide gels. Upper bar denotes the position of pro-VWF, lower bar denotes the position of mature propeptide cleaved VWF.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/2/10.1182_blood.v96.2.560/5/bloo01401003w.jpeg?Expires=1722684517&Signature=qCpweU8P5N2KRmB7gDJnQdG8lr~qbrQdS51OE2pJ2htyCS27yKNgAVfrW1-sAmth3RVfXME8g8GpncX7FIJotUFqxRLxxNoa1zIu8iZOKGpC7SsjzZ3-bu~wJCjbnyJQ4Ea-pJn8VknSLFjlSHJnfBT6AMeFLVnfR6GOKAzyWhrUslJC-AcVwThNL6kgvhXz4vitw7mH3W4X9B0MZN-6CzcnhfFPJya0u84isTtFhX7u59TCdgha30JR01FfqhNCsTL3NFBiPjerwhlngYyiJlIaC-KMKTZt4HANaNPYY8jHFn43WtBVzShD0p-Wo2RTpUm-7HTFkwYmhL86K8MFvw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of rVWFR273W secretion from COS-7 cells.
COS-7 cells were transfected with the appropriate plasmid DNA. Forty-eight hours after transfection, the cells were radiolabeled for 20 minutes with [35S]-methionine and [35S]-cysteine, then chased for various times in the presence of nonradioactive methionine and cysteine. Radiolabeled cell lysates and medium samples were immunoprecipitated with a rabbit polyclonal antiserum to VWF. Immunoprecipitates were separated by electrophoresis on 6% reducing SDS-polyacrylamide gels. Upper bar denotes the position of pro-VWF, lower bar denotes the position of mature propeptide cleaved VWF.
