American Society of Hematology

Fig. 2.

$Fig. 2. Steady-state analysis of rVWFR273W expressed in COS-7 cells. / The cells were transfected with the appropriate plasmid DNA, then placed in serum-free medium 24 hours after transfection. Cell lysates and medium supernatants were harvested after an additional 48 hours and the quantities of VWF in each fraction were measured by ELISA. Each bar represents the average values with Sds of 4 transfections and are expressed as percentages relative to wild-type VWF in 1 mL of lysate (26 mU ± 1 mU, n = 4) or 5 mL medium (69 mU ± 10 mU, n = 4). Wild-type is indicated by ▪, Arg273Trp is indicated by □.$

Steady-state analysis of rVWFR273W expressed in COS-7 cells.

The cells were transfected with the appropriate plasmid DNA, then placed in serum-free medium 24 hours after transfection. Cell lysates and medium supernatants were harvested after an additional 48 hours and the quantities of VWF in each fraction were measured by ELISA. Each bar represents the average values with Sds of 4 transfections and are expressed as percentages relative to wild-type VWF in 1 mL of lysate (26 mU ± 1 mU, n = 4) or 5 mL medium (69 mU ± 10 mU, n = 4). Wild-type is indicated by ▪, Arg273Trp is indicated by □.

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