Pedigrees of and multimer analysis of VWF in plasma from families.

(i) Pedigrees of families A (A), B (B), and C (C). Squares denote males, circles denote females. Slashed symbols represent deceased family members. Inheritance of the intron 40 VNTR-1 is shown as number of ATCT repeat units.32,33 (ii) Multimer analysis of VWF in plasma from members of families A, B, and C. Plasma samples were electrophoresed on 2% SDS-agarose gels. In A-C, lanes 1-3 contain equal volumes of plasma (2 μL). In A and B, lanes 4 and 5 contain the appropriate volume of plasma containing 0.5 mU VWF as determined by ELISA. VWF was transferred onto nitrocellulose by electroblotting. In A-C, lanes 1-3 were developed using polyclonal rabbit antihuman VWF antibody and a secondary alkaline phosphatase-conjugated swine antirabbit IgG polyclonal antibody and colorimetric staining. In A and B, lanes 4 and 5 were developed using rabbit antihuman VWF polyclonal antibody and a secondary horseradish peroxidase-conjugated swine antirabbit IgG polyclonal antibody and enhanced chemiluminescence. NP denotes plasma pooled from 20 healthy individuals.