Fig. 4.
Normal and mutant fVIII translation efficiencies.
CHO cells transiently transfected with normal and mutant fVIII cDNA expression vectors were labeled with [35S]methionine for 30 minutes. Cell extracts were analyzed by immunoprecipitation with a pool of monoclonal antibodies to fVIII light and heavy chains. Molecular weight markers are shown on the left. One experiment representative of 3 separate evaluations is shown.
