GST/4.1R-80–treated lysates exhibit reduced in vitro translation activity in a dose-dependent manner.

(A) Affinity-purified GST/4.1R-80 fusion protein (0.25 to approximately 2.0 μg) was visualized by Coomassie blue staining. (B) Rabbit reticulocyte lysates were incubated with the indicated amounts of soluble GST/4.1R-80 fusion protein for 2 hours at 4°C and tested for in vitro translation activity, as described in Figure6B. (C) Rabbit reticulocyte lysates were incubated with (lane 2) or without (lane 1) affinity-purified GST protein (2 μg) and tested for in vitro translation activity. In vitro translation activity was not inhibited by GST (C, lane 2) but was inhibited by the addition of GST/4.1R-80 fusion protein in a dose-dependent manner (B).