Characterization of anti-eIF3-p44 antibody.

The production of antibody against eIF3-p44 is described in “Materials and methods.” SiHa cells were transiently transfected with a FLAG-tagged eIF3-p44-1 plasmid. Cell lysates (10 μg) prepared from transfected cells (lane 1), untransfected cells (lane 2), and Molt-4 cells (lane 3) were separated by SDS-PAGE, transferred to a membrane, and immunoblotted with anti-FLAG antibody (A) or anti-eIF3-p44 antibody (B). The same blot was reprobed with an antibody against β-actin as a control. Positions of the molecular weight markers are shown on the left.