SIV infection of homozygous ccr5▵32 PBLs.

We stimulated ccr5Δ32 PBLs with OKT3/IL-2. (A) One week after stimulation, the indicated pseudotyped GFP reporter viruses were used to infect the stimulated cells in the presence or absence of the indicated antibodies (10 μg/mL anti-CD4 [leu3A], 20 μg/mL anti-CCR5^2D7, and 20 μg/mL anti-STRL33 [mAb 699]). The cells were fixed and analyzed by FACS for GFP expression 3 days later. (B) Pseudotyped GFP reporter viruses were also used to infect CD4- and CCR5- or STRL33-transfected 293T cells in the presence or absence of 20 μg/mL of the indicated antibodies (anti-CD4 [leu3A], 2D7 [R5 mAb], and anti-STRL33 [mAb 699]). The cells were harvested, fixed, and analyzed for GFP expression 2 days later. The results are presented as the percent of infected cells (GFP⁺) normalized against the negative control (percent of infected cells in the presence of 20 μg/mL mouse IgG). (C) Infected ccr5Δ32 PBLs from panel A were costained with anti-STRL33. GFP⁺ cells can clearly be seen in the STRL33⁺ gate (compare with uninfected/isotype control, at far right dot-plot). Data shown is representative of results from 2 donors.