Fig. 5.
![Fig. 5. DC–IFN-β have reduced capacity to induce a mixed lymphocyte reaction. / Naive CD4+ T cells were stimulated with either allogeneic DC or DC–IFN-β (0.1 ng/mL or 1 ng/mL) at the indicated ratios. DC were washed to remove cytokines used for in vitro differentiation. No exogenous cytokines were added to cultures for measurement of [3H]-thymidine incorporation. After 5 days in culture, wells were pulsed with 1 μCi of [3H]-thymidine per well. Twenty-four hours later, plates were harvested and incorporated [3H]-thymidine was measured. Means of triplicate measurements are shown. A representative of 4 independent experiments is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.210/4/bloo01352005x.jpeg?Expires=1724243575&Signature=1Kon80fSKJ6yOz3joPs3UIq9WrCbJt5u2hpzxlgGOiXX5agTCwQwd3s~dpICmvbLqbJ3PBrKgCUSS3HkdZ0ljDrptkrLVU4A7W~cxcTS~3NBoHgpL2djUn5QiFktwuRzn9K~sp24Y~9yfyNclafWk9E7vfHNX-m0vXezA5L7RsCoV-yNMwhw-rLA0bTCOhcIuC3PygtB5hpUhkG-90QtXUg93Jz1Sfuedeau16UIyWseMIDQSG5bB2cQ2~DKaCsJC8MRaE2WdDHXnFld2SLW9sCDTBdFtrpJDouIFYVXoJDI5qxhZBSzQqiryAaX6buOp6kkKESzJx1M7j9qYuAz3A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
DC–IFN-β have reduced capacity to induce a mixed lymphocyte reaction.
Naive CD4+ T cells were stimulated with either allogeneic DC or DC–IFN-β (0.1 ng/mL or 1 ng/mL) at the indicated ratios. DC were washed to remove cytokines used for in vitro differentiation. No exogenous cytokines were added to cultures for measurement of [3H]-thymidine incorporation. After 5 days in culture, wells were pulsed with 1 μCi of [3H]-thymidine per well. Twenty-four hours later, plates were harvested and incorporated [3H]-thymidine was measured. Means of triplicate measurements are shown. A representative of 4 independent experiments is shown.
