Effect of γδ T-cell depletion on pamidronate-induced autologous plasma cell decrease in BMMC cultures.

BMMC of 3 patients with MM (patients 1-3) were cultured under standard conditions (medium with 10 U/mL IL-2) or after depletion of γδ T cells by MACS (patients 1-3 γδ⁻) in the presence of different pamidronate concentrations (0.4 μmol/L, 4 μmol/L, 40 μmol/L). After 5 days, the number of viable plasma cells was determined as described in “Materials and methods.” Results are expressed as percentage of plasma cells according to the following calculation: [plasma cell number in pamidronate-treated cultures]/[plasma cell number in control cultures (medium alone)] × 100. Each bar represents the mean value ± SD of triplicate cultures.