Induction of CD25 (IL-2 receptor  chain) expression on BM γδ T cells of patients with MM.

BMMC of patients with MM were incubated with IPP (4 μmol/L) or pamidronate (4 μmol/L), and the relative cell number of CD25⁺ γδ T cells was determined by 2-color FACS analysis using anti-CD25 and anti-γδ TCR mAb after 72 hours of culture. Solid areas represent the fluorescence distribution of CD25 expression after gating on γδ TCR⁺ cells in 3 representative patients with MM.