Sensitivity of heparin-enhanced adhesion to trypsin.

With use of the parasite line A4, IE adhesion to resting HDMEC in the absence of any additive (□) or the presence of 100 μg/mL heparin () or 100 μg/mL chondroitin sulfate (▪) was compared in 3 sets of synchronized cultures that had been mock trypsinized, pretrypsinized during early ring-stage development, or trypsinized immediately before use during late-trophozoite-stage development. Results are the mean ± SD value for triplicate points from a single experiment.