MDAY-D2 cells transfected with the G208A mutant of the G11 protein, coexpressing EGFP.

G208A-G11: Bulk population of transfected parental cells. S1-G208A-G11: Cells transfected with both the S1 catalytic subunit of pertussis toxin and the G11 mutant (S1-G208A-G11). Clones 3 and 71 of the G208A-G11 transfectants were selected for their high GFP expression. (A) FACS profiles of GFP expression. The median fluorescence in arbitrary units is indicated. Untransfected MDAY-D2 cells serve as negative controls. (B) Expression of G11 in these populations and clones, as detected by Western blot analysis with a pAb.