Effect of SDF-1 and MDC on PGI₂-stimulated adenylyl cyclase activity.

Washed platelets were stimulated with 10 nmol/L carbacycline, a stable analog of PGI₂, resulting in an elevated level of cAMP in the treated platelets (represented as 100%). ADP (1 μmol/L) inhibited 70% of this increase. Similarly, 100 nmol/L SDF-1 inhibited 60% of the increased cAMP levels, while the same concentration of MDC had no significant affect on this assay. Data presented is the mean ± SEM for 5 experiments.