Studies examining whether the messages of either SDF-1 or MDC are detectable in platelet total RNA.

Results are given for (A) RT-PCR of human platelets and (B) RT-PCR of stromal cells. The expected size for each band is indicated. Lane 1: An SDF-1 message (281 bp) is present in stromal cells but not in platelets. Lane 2: An MDC message (270 bp) is also absent in platelets. Lanes 3-5 represent the following messages: Lane 3: A RANTES message (238 bp); Lane 4: An IL-8 message (536 bp); and Lane 5: An MIP-1α message (254 bp). Lane 6: The LFA-1 message (353 bp) is a white cell–specific message showing that the preparation is clean of contaminating white cell messages at the level of RT-PCR examined. Line 7: The ubiquitous message from β-actin (309 bp) was used as a general positive control for the RT-PCR studies.