Fig. 1.
Platelet aggregation using chemokine agonists.
(A) Platelet aggregation data using human washed platelets. Platelets were stimulated with 100 nmol/L chemokine (as shown) or 10 μmol/L ADP in the presence of 1 mmol/L CaCl2 and 200 μg/mL fibrinogen. (B) Ca++–flux data using Fura-2–loaded washed platelets involving 30 nmol/L MDC activation. This activation was inhibited by prior incubation of the platelets with 20 U/mL apyrase, as shown in the middle of the figure. The activation specificity is shown by the ability of 20 μg/mL anti-MDC antibody to block MDC-induced platelet activation, as shown on the right side of the figure.
