American Society of Hematology

Fig. 7.

$Fig. 7. The short eALAS is located in the mitochondrial membrane. / Blood (4 mL) was separated on a Ficoll-Hypaque gradient. The high-density fraction was collected and labeled with 22.2 MBq (600 μCi) 35S-methionine for 3 hours. Suborganelles were prepared, and the lysates were used for immunoprecipitation of the eALAS protein followed by gel separation. Lysates of membrane and matrix fractions contained 270 μg protein each.$

The short eALAS is located in the mitochondrial membrane.

Blood (4 mL) was separated on a Ficoll-Hypaque gradient. The high-density fraction was collected and labeled with 22.2 MBq (600 μCi) ³⁵S-methionine for 3 hours. Suborganelles were prepared, and the lysates were used for immunoprecipitation of the eALAS protein followed by gel separation. Lysates of membrane and matrix fractions contained 270 μg protein each.

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