Proteolytic cleavage of in vitro translated eALAS.

In vitro translated eALAS proteins representing the 2 natural splice variants pre–ALAS-E minor and pre–ALAS-E major were incubated alone (lanes 1 and 2) or with a bone marrow (b.m.) mitochondrial lysate (lanes 3 and 4) for 60 minutes at 27°C before gel separation. A labeled blood lysate was included as a control (lane 5).