Fig. 1.
eALAS protein synthesis in different erythroid tissues.
Peripheral blood and bone marrow were diluted with DMEM without methionine to 3 mL, separated on 3 mL Ficoll-Hypaque gradients, resuspended in 1 mL medium (blood, 1.5 × 108 cells; bone marrow, 1.9 × 107 cells), and incubated with 7.4 MBq (200 μCi) 35S-methionine for 3 hours. Detergent lysates were prepared, and eALAS and gel separation were immunoprecipitated. The arrows indicate the presumed pre-eALAS, the 59-kd form, and the herein described 52-kd eALAS-short.
