Flow cytometric analysis of spontaneous apoptosis and cytokine effects in leukemic cells from a patient with cortical T-ALL.

Blast cells were either cultured in medium alone or treated with 25 ng/mL rIL-7 for 24 hours. Freshly isolated (day 0) and cultured (24 hours; 24 hours +IL-7) cells were stained with annexin V-FITC and PI to differentiate between viable (lower-left quadrant) and apoptotic (lower- and upper-right quadrants) cells. Data shown are representative of T-ALL responsive to IL-7.