NF-E2 interacts directly with endogenous HS2 in living MEL cells.

(A) Ethidium bromide-stained agarose gels of input chromatin (left panel) and PCR products of samples using primers specific for mouse HS2 and the Jagged-1 gene from a representative experiment. The immunoprecipitation conditions are indicated below each lane. No antibody, No Ab; anti-p45 polyclonal sera, p45; preimmune sera, PI; no chromatin, No Chr. (B) Relative intensity of PCR products from 4 independent experiments (mean ± SEM). The signal obtained with 0.06% of input was set to 1.