Effect of IFN-γ on expression of Fas.

Data show typical results of 3 independent experiments. Panel (A) shows the baseline expression of Fas on day 7 ECFCs, prior to serum-free liquid culture. Cells were incubated with or without rhEPO (10 U/mL) or rhIFN-γ (1000 U/mL), or both, for 16 hours. Cells were then incubated on ice with FITC-Fas or FITC-murine IgG1 as control for 30 minutes, and then flow cytometric analysis was performed. The open histogram indicates murine IgG1 as control, and the gray histogram indicates Fas. Each panel shows data on cells incubated without rhEPO and rhIFN-γ (B), with rhEPO alone (C), with rhIFN-γ alone (D), and together with rhEPO plus rhIFN-γ (E).