Fig. 6.
![Fig. 6. [CD4XL/CD3] treatment of PBTs results in induction of TNF and IFNγ in T cells undergoing apoptosis; anti-CD28 stimulation results in IL-2 expression and rescues T cells from apoptosis. / PBTs (1 × 106/mL) were cultured in complete medium, or treated with CD4XL, and were stimulated after 24 hours with anti-CD3 mAb with or without CD28-stimulation in the presence of Brefeldin A. Following a culture period of 18 hours, cells were harvested and labeled with annexin V and anticytokine antibodies for simultaneous assessment of intracytoplasmic cytokine production and apoptosis in CD4 T cells (A) and in CD8 T cells (B). Percentages of positive cells are indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.195/4/bloo01351006x.jpeg?Expires=1724257920&Signature=nq1gnh4i96zW3NCf6mnHbuo9LZ2Uw~AiDgY3FjCPib~XeVOmVsjaqPSUp9FYkZ4ip5fRK-7Alkp8jyt1INUZC5FEnhnu5R41mj8Ril80TBLgxzmQTwYm3BIZhZCXCIOU2b2voWQDcxoCSWZYFTUwOrD3q2HqODXYQfxQPmTNelVk9Jqau2Ap-W1vkWpxUVMGfV0j0cdaajsaMiUetEpcKNcL3U0wSejWRAKtJnpKGZeR2dN6KMxy91AMzbFB4eKKq~H9cEJSkouYURZwOY~54H~yXl1SZpeKhCnWWGhILbK-qgsNWpPzTy9YojNJNG-4bEWAB4dCB909~UOtmWdU8A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
[CD4XL/CD3] treatment of PBTs results in induction of TNF and IFNγ in T cells undergoing apoptosis; anti-CD28 stimulation results in IL-2 expression and rescues T cells from apoptosis.
PBTs (1 × 106/mL) were cultured in complete medium, or treated with CD4XL, and were stimulated after 24 hours with anti-CD3 mAb with or without CD28-stimulation in the presence of Brefeldin A. Following a culture period of 18 hours, cells were harvested and labeled with annexin V and anticytokine antibodies for simultaneous assessment of intracytoplasmic cytokine production and apoptosis in CD4 T cells (A) and in CD8 T cells (B). Percentages of positive cells are indicated.
