Fig. 4.
![Fig. 4. CD3 stimulation of PBTs 24 hours after CD4XL down-modulates Bcl-x in both CD4 T cells and CD8 T cells, and CD28 costimulation prevents Bcl-x down-modulation. / [CD4XL/CD3] PBT cell cultures are showing Bcl-x expression in CD4 T cells (A) and in CD8 T cells (B). Cell-culture conditions are indicated in each panel, and isotype controls are indicated in top panels. Cells were analyzed by flow cytometry for intracytoplasmic Bcl-x expression in conjunction with surface labeling with FITC-anti-CD8 mAb. Broken lines represent the lowermost boundary of cells cultured in medium alone and labeled with anti–Bcl-x Ab. Cells that emerged to the left of this line represent Bcl-x down-modulated cells, and their percentages are indicated in the figure.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.195/4/bloo01351004x.jpeg?Expires=1724256786&Signature=KiZ2gHMhu~xfD1XE9-e~ZQOWdYuoDMPVdTbSnphXISpFwWeADBr3nLG4tBBCDZWq53jOhdm9t9dua0KWTzODLmUX09LhhhsE6aPBkyn2XtIHIUJT5VRC94~AQWyujC8NSE4vSvPU-PwJiyjGGaplGieK6PVIQVjrgtlkq9UbmSNqERFh~zKmnIIGDhcaVQatvG-9fKZY~jMSLN9IlXMUB4GiQJ118YwkcXiY1J5uqM9lHMLLizYFmZnVH-n42hE~IjXNT5NtidmjAaBP7w3YFyFQ4z1VzsFobiFWbPamX6qxzKcxRNGNjt3DNl-caH3i7aH1NxjJ5Y0HsLCvZZivIA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CD3 stimulation of PBTs 24 hours after CD4XL down-modulates Bcl-x in both CD4 T cells and CD8 T cells, and CD28 costimulation prevents Bcl-x down-modulation.
[CD4XL/CD3] PBT cell cultures are showing Bcl-x expression in CD4 T cells (A) and in CD8 T cells (B). Cell-culture conditions are indicated in each panel, and isotype controls are indicated in top panels. Cells were analyzed by flow cytometry for intracytoplasmic Bcl-x expression in conjunction with surface labeling with FITC-anti-CD8 mAb. Broken lines represent the lowermost boundary of cells cultured in medium alone and labeled with anti–Bcl-x Ab. Cells that emerged to the left of this line represent Bcl-x down-modulated cells, and their percentages are indicated in the figure.
