CD3 stimulation induces CD95L mRNA in CD4 T cells after priming with CD4XL.

Purified CD4 T cells (top) or CD8 T cells (bottom) were either untreated (medium: left panels) or CD4 cross-linked for 40 minutes (middle panel) or for 24 hours (right panel) and then stimulated with anti-CD3 mAb. Cells were harvested at the indicated times (in minutes) after addition of anti-CD3 mAb and examined for CD95L mRNA by RT-PCR. Figures show agarose gel electrophoresis stained with ethidium bromide for amplified fragments for CD95L and β-actin. Data represent 1 of 2 experiments.