Fig. 4.
![Fig. 4. Neutralization of endogenous IL-10 during LPS priming completely ameliorates the ET-associated suppression of T-cell IFN-γ production but not the suppression of T-cell proliferation in response to different protein recall antigens. / PBMCs from 2 donors were cultured for the first 24 hours with (2 ng/mL) or without LPS in the presence of either a control murine IgG1 or anti-IL-10 MoAb and recultured with either 2.5 μg/mL candidin (Can), 0.08 IU/mL tetanus toxoid (TT), 5 PPD-S/mL tuberculin (Tbc), or without stimulation. (A) Cellular [5′-3H]thymidine incorporation after 3 days of antigen stimulation is given from experiments in triplicate assays (mean ± SEM). (B) IFN-γ production after 4 days of antigen stimulation was assessed by ELISA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.218/4/bloo01304004x.jpeg?Expires=1724236173&Signature=QYfBZhrYtlIdI5rJuIm11d8JP6Gsdmy59bzSvK57rrQJP0pX-5qsJGeAHqXQp2kJAye~SjgvTtUTP5pXfYHv9kyd2VQiXkm9cC2PWuUi82B0orGt7eQd3C8X-~2bJQBJJK0zHCIb0~Fozqa~ZSAo3GcYH~mAUbau8TMbe7o9~QBkRMQ-kzTtKYl0-XdIq092PJVBj7OurSv6i2Qza7Tpxvbvk4mLdWAkbMQPJWsGjSTZMGcDzNUk7ldLJK5BYVgjXIcn3ApHSqye430cv7HFGjK-LgLbdwBWH~EAkh1s29R1FEt1ZC5dZ-atYYu6HgJ~AJcslg3ni4Myr9rmJKsY7Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Neutralization of endogenous IL-10 during LPS priming completely ameliorates the ET-associated suppression of T-cell IFN-γ production but not the suppression of T-cell proliferation in response to different protein recall antigens.
PBMCs from 2 donors were cultured for the first 24 hours with (2 ng/mL) or without LPS in the presence of either a control murine IgG1 or anti-IL-10 MoAb and recultured with either 2.5 μg/mL candidin (Can), 0.08 IU/mL tetanus toxoid (TT), 5 PPD-S/mL tuberculin (Tbc), or without stimulation. (A) Cellular [5′-3H]thymidine incorporation after 3 days of antigen stimulation is given from experiments in triplicate assays (mean ± SEM). (B) IFN-γ production after 4 days of antigen stimulation was assessed by ELISA.
