Fig. 3.
![Fig. 3. The capacity of monocytes to stimulate T-cell DNA synthesis in response to the protein recall antigen candidin is diminished during ET; study of different kinetics. / PBMCs were cultured in the presence or absence of 2 ng/mL LPS. Cells were washed and recultured at 24 hours with (2.5 μg/mL) or without candidin. After the time periods indicated, cells were pulsed with [5′-3H]thymidine for a further 15 hours. Results of the cellular radioactive labeling are presented as specific cpm (the difference between cpm of culture in the presence of antigen and cpm of culture in the absence of antigen). Representative data from 1 of 3 independent experiments in triplicate assays (mean ± SEM) are given.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.218/4/bloo01304003x.jpeg?Expires=1724235807&Signature=KuPga~XEfUDx3QyOeDlvXm0fX00lyOR48CXYCHDU0ydm-INCF4veAPDVBgawyQQQasMuxTHVUYTWEwqY1JAFhwv6skxGHgSa94jGX80quGIaHOoApl87JCrppeCrXmw1xbtWjHk9Qsbr5W8QhiIZO7~rdjV5DeMJ-PTF5HrnxfaJkFokl2Oo2bcb1b3vT08Jpof3Y2kUHMa4pTQqopNPQIMW9F5TT6DBYfi8yik6dJZvD8u0DncoFhuQxB4hn7uxyThkpH-aBqRr9uq1LMkm8wTQpakgvD1Kyziqy7FJuLr9a4m~Ix5vvFnsV~4Da1bXABK5ksJrpLDCsJ4YMTTCWA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The capacity of monocytes to stimulate T-cell DNA synthesis in response to the protein recall antigen candidin is diminished during ET; study of different kinetics.
PBMCs were cultured in the presence or absence of 2 ng/mL LPS. Cells were washed and recultured at 24 hours with (2.5 μg/mL) or without candidin. After the time periods indicated, cells were pulsed with [5′-3H]thymidine for a further 15 hours. Results of the cellular radioactive labeling are presented as specific cpm (the difference between cpm of culture in the presence of antigen and cpm of culture in the absence of antigen). Representative data from 1 of 3 independent experiments in triplicate assays (mean ± SEM) are given.
