American Society of Hematology

Fig. 6.

$Fig. 6. HLA restriction pattern of EBV-reactive T cells of donor U.S. / The pattern is shown (A) after 12 days in culture, (B) after 30 days in culture, and (C) in the NIT+ fraction after gene transfer on day 8 and FACSort on day 11. In the 51Cr release assay, each donor MHC class I allele could be paired with a BLCL, expressing that allele homozygously. HLA-B*4403 is the allele, presenting predominantly EBV peptide to the T cells in the late culture, as opposed to the broader HLA spectrum of T cells cultured for 12 days only. The more restricted pattern to HLA-B*4403 can be demonstrated in the NIT+ fraction after early selection.$

HLA restriction pattern of EBV-reactive T cells of donor U.S.

The pattern is shown (A) after 12 days in culture, (B) after 30 days in culture, and (C) in the NIT⁺ fraction after gene transfer on day 8 and FACSort on day 11. In the ⁵¹Cr release assay, each donor MHC class I allele could be paired with a BLCL, expressing that allele homozygously. HLA-B*4403 is the allele, presenting predominantly EBV peptide to the T cells in the late culture, as opposed to the broader HLA spectrum of T cells cultured for 12 days only. The more restricted pattern to HLA-B*4403 can be demonstrated in the NIT⁺ fraction after early selection.

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