American Society of Hematology

Fig. 1.

$Fig. 1. Cell purification by dual-color FACSort. / (A) Monitoring of gene expression (19.3%) 3 days after transduction of NIT. (B) CD3+ and NGFR+ T cells, termed NIT+ fraction, and (C) CD3+ and NGFR−, termed NIT− fraction, were sorted to high purity, 97.52% and 99.54%, respectively, in this example. Purity of sorted cell fractions was above 95% in all experiments. Cells of the unsorted fractions were included in the functional assays.$

Cell purification by dual-color FACSort.

(A) Monitoring of gene expression (19.3%) 3 days after transduction of NIT. (B) CD3⁺ and NGFR⁺ T cells, termed NIT⁺ fraction, and (C) CD3⁺ and NGFR⁻, termed NIT⁻ fraction, were sorted to high purity, 97.52% and 99.54%, respectively, in this example. Purity of sorted cell fractions was above 95% in all experiments. Cells of the unsorted fractions were included in the functional assays.

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