Fig. 3.
Inhibition of biotin-HKa binding to Mac-1 transfected cells by HKa.
Biotin–HKa (10 nmol/L) was incubated with Mac-1 transfected HEK 293 cells (4 × 106/mL) in binding buffer containing 50 μmol/L ZnCl2 for 45 minutes at room temperature in the presence of indicated HKa concentrations. The fluorescence of cell-bound biotin–HKa was measured, and the percentage of biotin–HKa bound to the cells in the presence of HKa was determined by comparing that with biotin–HKa alone, which was 100%. Data are the mean ± SE of 3 separate experiments.
