HTLV-I-infected T-cell lines.

(A) Determination of HTLV-I mRNA expression by Northern blot analysis in HTLV-I-infected T-cell lines. GAPDH expression was used as a control. Predominant 2.1, 4.2, and 8.5 kb viral mRNA species were detected in MT-2, MT-4, C5/MJ, SLB-1, and HUT-102 cell lines (lanes 4-8). (B) Expression of Tax protein in HTLV-I-infected T-cell lines determined by Western blotting using the monoclonal antibody, Lt-4. (C) Increased AP-1 binding activity in HTLV-I-infected T-cell lines. Labeled IL-8 AP-1 oligonucleotide was incubated with nuclear extracts of T-cell lines and the mixture was separated by polyacrylamide gel. Jurkat, MOLT-4, and CCRF-CEM are HTLV-I-negative cells and the remaining cell lines are HTLV-I infected. (D) Sequence specificity of AP-1 binding activity in ATL-derived cell line, MT-1. The binding reaction was carried out in the presence of 100 ng of the indicated cold oligonucleotides as competitors.