Fig. 4.
Fig. 4. Ribonuclease protection assay of AG-490–treated D10 cells does not result in altered mRNA expression of γ chain receptors. / Complementary DNA was generated from freshly isolated mRNA obtained from 2 sets of D10 cells treated in the absence (lanes A and B) or presence (lanes C and D) of 100 μmol/L AG-490 for 16 hours. D10 RNA was then probed with 33P-labeled RNA probes corresponding to transcripts for individual murine γ chain receptors (mCR-1), according to the PharMingen protocol. The autoradiographs of the RNase-protected fragments were separated by 5% polyacrylamide gel electrophoresis.

Ribonuclease protection assay of AG-490–treated D10 cells does not result in altered mRNA expression of γ chain receptors.

Complementary DNA was generated from freshly isolated mRNA obtained from 2 sets of D10 cells treated in the absence (lanes A and B) or presence (lanes C and D) of 100 μmol/L AG-490 for 16 hours. D10 RNA was then probed with 33P-labeled RNA probes corresponding to transcripts for individual murine γ chain receptors (mCR-1), according to the PharMingen protocol. The autoradiographs of the RNase-protected fragments were separated by 5% polyacrylamide gel electrophoresis.

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