Fig. 5.
p53 accumulation in both B-CLL and normal lymphocytes after MG132.
Western blot analyses of the nuclear wild-type p53 at different times after MG132 (1 μmol/L) treatment of PBL or B-CLL cells versus untreated cells (time 0). An equal amount of protein (derived from 1.5 × 106 cells) was deposited in each lane. The same accumulation was obtained with LLnL (25 μmol/L) in both cell types. The results represent analyses of cells from 10 different patients and 10 different normal donors.
