Comparing the performance of the V_H3–Fr2 and V_H3–Fr3 probe in real-time quantitation.

Bone marrow from patients H and 6 were subjected to real-time IgH ASO–PCR in 5-fold dilutions. Using these results, calibration curves were constructed by plotting the initial number of tumor cells against the threshold cycle (±SD). The V_H3–Fr3 probe in patient H has 1 mismatch.