Quantitating dilution series of tumor cells of patient H in dH₂O using real-time ASO–PCR.

Squares and triangles show the average Ct (±SD) of 3 quantitation experiments with the V_H3–Fr2 and V_H3–Taqman probe, respectively. Quantitation of the absolute number of cells was performed using β-actin real-time PCR (diamonds).