Proteasome inhibitors increase FANCC expression.

293 FANCC E2 cells were treated with media only (Control) or media supplemented with vehicle alone (DMSO), 20 μm MG132 (MG132), or 10 μmol/L lactacystin (LACT.) for 24 hours in the presence or absence of 1.3 mmol/L HU. Whole-cell extracts were analyzed for FANCC expression by Western blotting and probing with monoclonal anti-FANCC antibody (8F3). Ten percent of the cells from each experimental condition were analyzed for DNA content by flow cytometry (Table 1). Arrow indicates full-length FANCC. The apparent increase in size of full-length FANCC in inhibitor-treated cells is an artifact of this particular gel (see also Figure 7).