Fig. 5.
Fig. 5. FANCC mRNA expression is constant during cell cycle progression. / (A) 293 NEO cells were synchronized with HU and released from the G1/S boundary as described above. Total cytoplasmic RNA was prepared from synchronized cells or from a culture of asynchronous 293 NEO cells (Asynch.). Endogenous FANCC and β-actin mRNA were analyzed by ribonuclease protection assay. A representative experiment is shown. (B) Identical experiments were performed using 293 FANCC E2 cells. Total FANCC (endogenous plus exogenous) and β-actin mRNA were analyzed by ribonuclease protection assay. A representative experiment is shown. Exposure times were identical for membranes depicted in A and B to demonstrate that 293 FANCC cells express more FANCC mRNA than 293 NEO cells.

FANCC mRNA expression is constant during cell cycle progression.

(A) 293 NEO cells were synchronized with HU and released from the G1/S boundary as described above. Total cytoplasmic RNA was prepared from synchronized cells or from a culture of asynchronous 293 NEO cells (Asynch.). Endogenous FANCC and β-actin mRNA were analyzed by ribonuclease protection assay. A representative experiment is shown. (B) Identical experiments were performed using 293 FANCC E2 cells. Total FANCC (endogenous plus exogenous) and β-actin mRNA were analyzed by ribonuclease protection assay. A representative experiment is shown. Exposure times were identical for membranes depicted in A and B to demonstrate that 293 FANCC cells express more FANCC mRNA than 293 NEO cells.

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