Results of in vitro and in vivo studies of endostatin.
(A) Specific inhibition of human umbilical vein endothelial cells (HUVEC) (⧫) by endostatin. HUVEC were cultured in medium 200 supplemented with 10 ng/mL vascular endothelial growth factor and basic fibroblast growth factor. Namalwa cells (▪) were cultured in RPMI–8% fetal bovine serum in a 72-hour proliferation assay.8 Endostatin inhibited proliferation of HUVEC but not Namalwa cells in a dose-dependent fashion. Results are mean ± SD values (n = 3). (B) Endostatin treatment (⧫, 50 μg per mouse on days 3 to 7) significantly delayed tumor growth in NOD/SCID mice given transplants of 10 × 106 Namalwa cells intraperitoneally. The dotted line indicates tumor growth in untreated controls (▪). Results are mean ± SD values for tumor volume (n = 4 per group). *P < 0.01.
