American Society of Hematology

Fig. 1.

$Fig. 1. Expression of MET receptor in peripheral blood mononuclear cells (PBMNC). / Immunocytologic detection of MET receptor in PBMNC (A) and monocytes (C) derived from a healthy donor by using a rabbit polyclonal MET antibody. The specificity of the signal was demonstrated by control experiments for PBMNC (B) and monocytes (D) using the MET antibody after preabsorption with a specific binding peptide. (E) Determination of monocyte concentrations by fluorescence-activated cell-sorter analysis using fluorescein isothiocyanate–labeled anti-CD14 (right) antibodies in the PBMNC fraction (above) and the negatively selected monocyte fraction (below). The respective unstained cell fractions are shown on the right side. The calculation resulted in a purity of the monocytic fraction of greater than 90%.$

Expression of MET receptor in peripheral blood mononuclear cells (PBMNC).

Immunocytologic detection of MET receptor in PBMNC (A) and monocytes (C) derived from a healthy donor by using a rabbit polyclonal MET antibody. The specificity of the signal was demonstrated by control experiments for PBMNC (B) and monocytes (D) using the MET antibody after preabsorption with a specific binding peptide. (E) Determination of monocyte concentrations by fluorescence-activated cell-sorter analysis using fluorescein isothiocyanate–labeled anti-CD14 (right) antibodies in the PBMNC fraction (above) and the negatively selected monocyte fraction (below). The respective unstained cell fractions are shown on the right side. The calculation resulted in a purity of the monocytic fraction of greater than 90%.

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