Plasmid recombination assay using construct pDVG93.

Transfection of pDVG93, RAG1, and RAG2 in CHO cells leads to an inversion rearrangement of pDVG93, which can be detected by primers DG89 and DG147 (A). PCR reactions with primers DG89 and DG147 were performed on serial dilutions of recombination products, as indicated. PCR products were visualized by hybridization with ³²P-labeled oligonucleotide FM23 followed by phosphor imaging. The N-terminal truncated RAG1 protein is still able to perform inversion rearrangement of pDVG93, although the activity may be slightly reduced as compared to wt RAG1 protein (B).