Agarose gel and heteroduplex PCR analysis ofTCR gene rearrangements.

TCRB gene rearrangements (RT-PCR with Vβ-Cβ primer) (A), TCRG gene rearrangements (B), and TCRD gene rearrangements (C) showing oligoclonal rearrangement patterns in an otherwise polyclonal background. The upper part of each panel shows the presence of PCR products in agarose gels for virtually each primer combination, whereas the lower part of each panel shows heteroduplex analysis of the obtained PCR products. The oligoclonal patterns are probably related to the high T-cell counts with expansion of several T-lymphocyte clones.