Sp1 and C/EBP cotransfection with LF89 inDrosophila S2 cells.

Drosophila S2 cells were cotransfected with 10 μg of wild-type LF89 plasmid and 5 μg of an Sp1 expression plasmid (pPAC-Sp1), 5 μg of a C/EBPα expression plasmid (pPAC-C/EBPα), or 5 μg of each expression plasmid. Salmon-sperm DNA was used to normalize the total amount of DNA used in each transfection. Transfected cells were harvested 48 hours after transfection. Normalized luciferase values (per microgram of protein) are represented as a ratio of enzyme activity of LF89 plus expression plasmid to enzyme activity of LF89 without expression plasmid. The mean ± SE value for 3 experiments performed in duplicate is represented.