Analysis of ghost and skeletal proteins in β-adducin mutant mice.

(A) Coomassie blue stained gels (SDS-PAGE) of ghosts and membrane skeletons (30 μg and 35 μg for ghost and skeleton, respectively) from normal (+/+) and mutant (−/−) mice. The major proteins are indicated. Variations between normal and mutant animals are indicated by arrows. (B and C) Western blot analysis of ghost proteins (3 μg and 8 μg, respectively) of adult wild-type (+/+), heterozygous (+/−), and homozygous mutant (−/−) mice using a rabbit anti-rat α-adducin polyclonal antibody (panel B) or a rabbit anti-mouse-γ-adducin antibody (panel C).