Fig. 3.
Fig. 3. Wright-Giemsa stain and TUNEL assay of PBMCs obtained from a patient 21 days following transplantation, incubated for 24 hours, and separated by sorting into CD3+/7AAD+and CD3+/7AAD− cells. / (A) Cells in the CD3+/7AAD+ fraction showed reduction in cell volume, blebbing of plasma membrane, chromatin condensation, and nuclear fragmentation (original magnification × 1000), (B) while cells in the CD3+/7AAD− fraction showed a typical morphological feature of lymphocytes. (C) Cells in the CD3+/7AAD+ fraction were brightly fluorescent, indicating incorporation of fluorescein-dUTP into fragmented DNA (original magnification × 400), (D) while cells in the CD3+/7AAD− fraction showed no significant fluorescence.

Wright-Giemsa stain and TUNEL assay of PBMCs obtained from a patient 21 days following transplantation, incubated for 24 hours, and separated by sorting into CD3+/7AAD+and CD3+/7AAD− cells.

(A) Cells in the CD3+/7AAD+ fraction showed reduction in cell volume, blebbing of plasma membrane, chromatin condensation, and nuclear fragmentation (original magnification × 1000), (B) while cells in the CD3+/7AAD− fraction showed a typical morphological feature of lymphocytes. (C) Cells in the CD3+/7AAD+ fraction were brightly fluorescent, indicating incorporation of fluorescein-dUTP into fragmented DNA (original magnification × 400), (D) while cells in the CD3+/7AAD− fraction showed no significant fluorescence.

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