Generation of probes for expression analysis of 2 different cellular transcripts.

The selected cellular DNA sequences flanking retroviral integrations were compared with gene sequences in the GenBank database. The sequence “50” is similar to the human putative RNA-binding protein RNPL (U28686). The sequence “134” overlaps with the 5′ end EST sequence T35609 similar to human alpha NAC mRNA (X80909). Respective fragments were used as probes in subsequent Northern blot analyses. ⇌ Oligonucleotides used to amplify fragments from TF-1 total RNA ▭ 5′RACE sequence; >>>>> EST sequence continue; ---- Mismatches between alignment, SA: Splice acceptor, open reading frame for the production of the related proteins.