Fig. 2.
Fig. 2. Effect of HHV-7 on CB CD34–derived granulocytic cultures. / Data are expressed as means ± SD of 4 to 6 independent experiments performed in duplicate. (A) Phenotypic analysis of surface CD34 and CD33, performed after 7 days of liquid culture supplemented with SCF + IL-3 ± G-CSF in HHV-7–infected and mock-infected cultures. The surface expression of CD34 and CD33 was analyzed by FACS and reported as the percentage of positive cells in cultures analyzed at the time indicated. (B) Phenotypic analysis of surface CD14 and CD15 performed after 7 to 14 days of liquid culture supplemented with SCF + IL-3 + G-CSF in HHV-7–infected and mock-infected cultures. The surface expression of CD14 and CD15 was analyzed by FACS and reported as the percentage of positive cells in cultures analyzed at the time indicated. (C) Viable cell counts performed at the time indicated by trypan blue dye exclusion.

Effect of HHV-7 on CB CD34–derived granulocytic cultures.

Data are expressed as means ± SD of 4 to 6 independent experiments performed in duplicate. (A) Phenotypic analysis of surface CD34 and CD33, performed after 7 days of liquid culture supplemented with SCF + IL-3 ± G-CSF in HHV-7–infected and mock-infected cultures. The surface expression of CD34 and CD33 was analyzed by FACS and reported as the percentage of positive cells in cultures analyzed at the time indicated. (B) Phenotypic analysis of surface CD14 and CD15 performed after 7 to 14 days of liquid culture supplemented with SCF + IL-3 + G-CSF in HHV-7–infected and mock-infected cultures. The surface expression of CD14 and CD15 was analyzed by FACS and reported as the percentage of positive cells in cultures analyzed at the time indicated. (C) Viable cell counts performed at the time indicated by trypan blue dye exclusion.

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