TEL-JAK2 leukemic cells express activated STAT1 and STAT5.

Electrophoretic mobility shift assays were carried out with either the m67SIE probe (upper panel) or the β casein probe (lower panel) and whole-cell extracts from the spleen and lymph nodes of a diseased transgenic animal (71-45) or from a control spleen. Positions of the STAT/DNA complexes are indicated by open arrowheads. Specific and nonspecific competitors were added as indicated. For supershift experiments, anti-STAT1 or anti-STAT5 antibodies were added as indicated. Supershifted complexes are indicated by black arrowheads.