: CD4 and CD8 expression in TEL-JAK2 leukemic cells.

(A) Single-cell suspensions from lymph nodes (LN), thymus (Th), spleen (Sp), and bone marrow (BM) were obtained from a transgenic leukemic animal (71-4) and its sibling control (71-2). (B) Single-cell suspensions obtained from a tumor generated in nude mice (Nu18; Table3) were sorted as CD8⁺ SP and CD4⁺CD8⁺ DP as described in “Materials and methods.” The CD4 and CD8 expression pattern of unsorted cells (left), CD8⁺ SP cells (middle), and DP cells (right) was determined by flow cytometry. For each experiment, 10⁶cells were incubated with anti-CD4–PE (Y-axis) and anti-CD8–FITC (X-axis) and analyzed by flow cytometry. The results were computed from the acquisition of 10 000 events. The relative percentage of each subpopulation (CD4⁺CD8⁻; CD4⁻CD8⁺; and CD4⁺CD8⁺) is indicated in the corresponding area of the diagrams.