Increased calcium flux in CD9-transfected myeloma cell lines.

Nontransfected and CD9-transfected myeloma cell lines COL, MER, and RLO were loaded with fluo-3/AM and placed in 8-chamber slides coated with poly-L-lysine. Both nontransfected and CD9-transfected cells were stimulated with anti-CD9 antibodies (ALB6 and MM2/57, 20 μg/mL), Ca⁺⁺ ionophore A23187 (5 μmol/L), and anti-CD38 antibodies (25 μg/mL). Data acquisition and analysis were performed on a Meridian ACAS 570 image analyzer. The data represent 4 to 5 of at least 20 cells observed with similar results from a total of 5 individual experiments.